iPSC reprogramming from human peripheral blood using Sendai Virus mediated gene transfer

Wenli Yang, Jason A. Mills, Spencer Sullivan, Ying Liu, Deborah L. French and Paul Gadue

This protocol allows efficient generation of integration-free iPS cells from a small amount of peripheral blood. Peripheral blood mononuclear cells (PBMCs) are cultured to expand the erythroblast (EB) population. They are then used to derive iPS cells using four recombinant Sendai viral vectors, expressing the four reprogramming factors Oct4, Sox2, Kfl4 and c-Myc.

iPSC derivation from fibroblast in chemically defined medium

Guokai Chen

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), have the potential to become the source materials for cell-based therapy, so the quality of the stem cells has great impact on how the cells could be utilized in future applications. Derivation and maintenance conditions have critical role determining the iPSC quality, mainly due to the involvement of animal products and feeder cells.

Blood - SeV derived fibroblast generated iPSCs

Laurence Daheron and Sunita D'Souza

CytoTune™-iPS Reprogramming System uses vectors based on replication in competent Sendai virus (SeV) to safely and effectively deliver and express key genetic factors necessary for reprogramming somatic cells into iPSCs. In contrast to many available protocols, which rely on viral vectors that integrate into the genome of the host cell...