Chondrocyte protocol

Rachel A. Oldershaw, Melissa A. Baxter, Emma T. Lowe, Nicola Bates, Lisa M. Grady, Francesca Soncin, Daniel R. Brison, Timothy E. Hardingham, Susan J. Kimber

We have developed for hESc a new 3-Stage directed differentiation protocol (DDP) to generate chondrocytes, the specialized cells that form cartilage tissue. The protocol is segmented into stages that mimic the developmental processes that occur in cell lineage specification during embryogenesis.